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Medium: CLOSTRIDIUM CELLOBIOPARUM medium_replace_Rumen fluid, clarified_with_Sludge fluid (medium 119)



Medium features

ID:140_replace_Rumen fluid, clarified_with_Sludge fluid (medium 119)
Name:CLOSTRIDIUM CELLOBIOPARUM medium_replace_Rumen fluid, clarified_with_Sludge fluid (medium 119)
PH Info: 6.8
Is Complex:true
Is Aerobic:true
Is SubMedium:false
Remarks:
Instructions:http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium140.pdf


Metabolites list

Compounds in the medium listed by their compound names. Gram & Mol amounts are per Liter of medium. Trace metabolites & gases usually do not have amounts

Name Semi unique name Unique Name Gram Amount Mol Amount
KH2PO4 Potassium dihydrogen phosphate SEED-cpd00009#cpd00205## 0.50 0.00
CaCl2 x 2 H2O Calcium chloride SEED-cpd00063#cpd00099## 0.10 0.00
Na2S x 9 H2O Sodium sulfide hydrate SEED-cpd00239#cpd00971## 0.25 0.00
Yeast extract Yeast extract rich-peptone# 1.20 null
K2HPO4 di Potassium hydrogen phosphate SEED-cpd00009#cpd00205## 0.50 0.00
Cellobiose Cellobiose SEED-cpd00158### 5.00 0.01
(NH4)2 SO4 (NH4)2 SO4 SEED-cpd00013#cpd00048## 0.50 0.00
MgSO4 x 7 H2O Magnesium sulfate SEED-cpd00048#cpd00254## 0.10 0.00
Distilled water H2O SEED-cpd00001### null null
Resazurin Resazurin SEED-cpd08053### 1.00E-3 4.37E-6
Cysteine-HCl x H2O Cysteine-HCl SEED-cpd00084#cpd00099## 0.25 0.00
NaCl NaCl SEED-cpd00099#cpd00971## 1.00 0.02
NaHCO3 sodium bicarbonate SEED-cpd00242#cpd00971#cpd09249# 10.00 0.12
sludge from an anaerobic digester Sludge fluid rich-rumen# 300.00 0.30


Seed Metabolites list

All SEED compounds in the medium, listed with molar concentrations (mol/L). Some SEED compounds appear in multiple compound names in the 'metabolite list' -- here, they are combined for final molar concentrations.

Seed ID Name Mol Concentration
cpd00242 H2CO3 0.11904761904761904
cpd00158 CELB 0.014619883040935672
cpd00971 Na+ 0.13834365214880012
cpd09249 Sodium bicarbonate 0.0
cpd00063 Ca2+ 6.835269993164729E-4
cpd00013 NH3 0.007575757575757576
cpd00048 Sulfate 0.004192655148939302
cpd00084 L-Cysteine 0.001435544071202986
cpd00001 H2O 0.7000000000000001
cpd00009 Phosphate 0.006577266922094508
cpd00099 Cl- 0.02004397738018076
cpd00239 H2S 0.001027326895418122
cpd00205 K+ 0.009450830140485313
cpd00254 Mg 4.047763610605141E-4
cpd08053 Resazurin 4.3668122270742355E-6

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