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Medium: METHANOSARCINA (thermophilic) medium



Medium features

ID:164
Name:METHANOSARCINA (thermophilic) medium
PH Info:null
Is Complex:true
Is Aerobic:false
Is SubMedium:false
Remarks:
Instructions:http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium164.pdf


Metabolites list

Compounds in the medium listed by their compound names. Gram & Mol amounts are per Liter of medium. Trace metabolites & gases usually do not have amounts

Name Semi unique name Unique Name Gram Amount Mol Amount
D-Ca-pantothenate D-Ca-pantothenate SEED-cpd00063#cpd00644## 4.66E-5 1.81E-7
KH2PO4 Potassium dihydrogen phosphate SEED-cpd00009#cpd00205## 0.21 0.00
CoCl2 x 6 H2O CoCl2 SEED-cpd00099#cpd00149## 1.77E-4 7.43E-7
Yeast extract (Difco) Yeast extract rich-peptone# 1.87 null
HCl HCl SEED-cpd00099### 6.27E-4 1.79E-5
CaCl2 x 2 H2O Calcium chloride SEED-cpd00063#cpd00099## 0.23 0.00
Na2S x 9 H2O Sodium sulfide hydrate SEED-cpd00239#cpd00971## 0.28 0.00
ZnCl2 ZnCl2 SEED-cpd00034#cpd00099## 6.51E-5 4.82E-7
Casitone casitone rich-peptone# 1.87 null
Pyridoxine HCl Pyridoxine HCl SEED-cpd00099#cpd00263## 9.33E-5 4.57E-7
Methanol Methanol SEED-cpd00116### null null
Thiamine-HCl x 2 H2O Thiamine-HCl SEED-cpd00099#cpd00305## 4.66E-5 1.39E-7
NH4Cl Ammonium chloride SEED-cpd00013#cpd00099## 0.47 0.01
CO2 CO2 SEED-cpd00011### null null
CuCl2 x 2 H2O CuCl2 SEED-cpd00058#cpd00099## 1.86E-6 1.10E-8
H3BO3 Boric acid SEED-cpd09225### 5.58E-6 9.00E-8
Na2MoO4 x 2 H2O Na2MoO4 SEED-cpd00971#cpd11145## 3.35E-5 1.38E-7
NiCl2 x 6 H2O NiCl2 SEED-cpd00099#cpd00244## 2.23E-5 9.39E-8
MgSO4 x 7 H2O Magnesium sulfate SEED-cpd00048#cpd00254## 0.47 0.00
H2O H2O SEED-cpd00001### null null
Resazurin Resazurin SEED-cpd08053### 9.33E-4 4.07E-6
Cysteine-HCl x H2O Cysteine-HCl SEED-cpd00084#cpd00099## 0.28 0.00
clarified rumen fluid clarified rumen fluid rich-rumen# 47.62 0.05
Folic acid Folic acid SEED-cpd00393### 1.87E-5 4.25E-8
Biotin Biotin SEED-cpd00104### 1.87E-5 7.68E-8
NaHCO3 sodium bicarbonate SEED-cpd00242#cpd00971#cpd09249# 1.90 0.02
K2HPO4 di Potassium hydrogen phosphate SEED-cpd00009#cpd00205## 0.32 0.00
MnCl2 x 4 H2O MnCl2 SEED-cpd00030#cpd00099## 9.30E-5 4.71E-7
FeSO4 x 7 H2O Iron sulfate SEED-cpd00048#cpd10515## 1.87E-3 6.69E-6
Nicotinic acid Niacin SEED-cpd00218### 4.66E-5 3.82E-7
N2 N2 SEED-cpd00528### null null
Lipoic acid Lipoic acid SEED-cpd00541### 4.66E-5 2.28E-7
p-Aminobenzoic acid p Aminobenzoic acid (PABA) SEED-cpd00443### 4.66E-5 3.43E-7
Riboflavin Riboflavin SEED-cpd00220### 4.66E-5 1.24E-7
Vitamin B12 Coenzyme B12 SEED-cpd00166### 9.33E-7 5.91E-10
Distilled water H2O SEED-cpd00001### null null
FeCl2 x 4 H2O Iron chloride SEED-cpd00099#cpd10516## 1.40E-3 7.03E-6
NaCl NaCl SEED-cpd00099#cpd00971## 2.10 0.04


Seed Metabolites list

All SEED compounds in the medium, listed with molar concentrations (mol/L). Some SEED compounds appear in multiple compound names in the 'metabolite list' -- here, they are combined for final molar concentrations.

Seed ID Name Mol Concentration
cpd00058 Cu2+ 1.099192142256685E-8
cpd00244 Ni2+ 9.386795948754438E-8
cpd00242 H2CO3 0.022675736961451247
cpd00220 RIBF 1.240415310892732E-7
cpd00030 Mn2+ 4.70883678350346E-7
cpd09249 Sodium bicarbonate 0.0
cpd00034 Zn2+ 4.824639288105176E-7
cpd00011 CO2 0.0
cpd00013 NH3 0.008799927488597495
cpd09225 Boric acid 9.004511113744682E-8
cpd00541 Lipoate 2.2751032043691084E-7
cpd00009 Phosphate 0.00343405762929106
cpd00239 H2S 0.001149939158156566
cpd00218 Niacin 3.8229193188169447E-7
cpd00263 Pyridoxol 4.5725113421143846E-7
cpd08053 Resazurin 4.073328881184866E-6
cpd00116 Methanol 0.009327923137913344
cpd00443 ABEE 3.4293835065857883E-7
cpd00149 Co2+ 7.431213459430599E-7
cpd00166 Calomide 5.90748773775386E-10
cpd00971 Na+ 0.0611618004925755
cpd11145 Sodium molybdate 1.38245073640653E-7
cpd00063 Ca2+ 0.0015941526017823747
cpd00528 N2 0.0
cpd10515 Fe2+ 6.68548513736846E-6
cpd00644 PAN 1.8077370422312683E-7
cpd10516 fe3 7.027689942751387E-6
cpd00048 Sulfate 0.0018945468771457358
cpd00084 L-Cysteine 0.0016068773708722386
cpd00001 H2O 0.943050703077668
cpd00305 THI 1.3868455453335332E-7
cpd00104 BIOT 7.677302994167363E-8
cpd00099 Cl- 0.04981682268688924
cpd00205 K+ 0.005299642256873729
cpd00254 Mg 0.0018878613920083674
cpd00393 FOL 4.249623297454827E-8

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