Medium: TREPONEMA SUCCINIFACIENS medium Medium features ID:275 Name:TREPONEMA SUCCINIFACIENS medium PH Info: 6.2 - 6.3, HCl Is Complex:true Is Aerobic:true Is SubMedium:false Remarks: Instructions:http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium275.pdf Metabolites list Compounds in the medium listed by their compound names. Gram & Mol amounts are per Liter of medium. Trace metabolites & gases usually do not have amounts Name Semi unique name Unique Name Gram Amount Mol Amount KH2PO4 Potassium dihydrogen phosphate SEED-cpd00009#cpd00205## 0.50 0.00 HCl HCl SEED-cpd00099### null null Yeast extract (Difco) Yeast extract rich-peptone# 0.50 null CaCl2 x 2 H2O Calcium chloride SEED-cpd00063#cpd00099## 0.10 0.00 K2HPO4 di Potassium hydrogen phosphate SEED-cpd00009#cpd00205## 0.50 0.00 Glucose D-Glucose SEED-cpd00027### null null N2 N2 SEED-cpd00528### null null (NH4)2SO4 Ammonium sulfate SEED-cpd00013#cpd00048## 0.50 0.00 Potassium phosphate buffer, /ph/ 7.4 Potassium phosphate buffer SEED-cpd00009#cpd00205## 3.37 0.02 MgSO4 x 7 H2O Magnesium sulfate SEED-cpd00048#cpd00254## 0.10 0.00 Distilled water H2O SEED-cpd00001### null null Peptone (Difco) Peptone rich-peptone# 0.50 null Resazurin Resazurin SEED-cpd08053### 1.00E-3 4.37E-6 Cysteine-HCl x H2O Cysteine-HCl SEED-cpd00084#cpd00099## 0.50 0.00 NaCl NaCl SEED-cpd00099#cpd00971## 1.00 0.02 clarified rumen fluid clarified rumen fluid rich-rumen# 300.00 0.30 NaHCO3 sodium bicarbonate SEED-cpd00242#cpd00971#cpd09249# null null Seed Metabolites list All SEED compounds in the medium, listed with molar concentrations (mol/L). Some SEED compounds appear in multiple compound names in the 'metabolite list' -- here, they are combined for final molar concentrations. Seed ID Name Mol Concentration cpd00242 H2CO3 0.0025 cpd00971 Na+ 0.019741379310344823 cpd09249 Sodium bicarbonate 0.0 cpd00063 Ca2+ 6.835269993164728E-4 cpd00027 D-Glucose 0.01 cpd00528 N2 0.0 cpd00013 NH3 0.007575757575757574 cpd00048 Sulfate 0.004192655148939301 cpd00084 L-Cysteine 0.002871088142405971 cpd00001 H2O 0.575 cpd00009 Phosphate 0.031577266922094505 cpd00099 Cl- 0.02147952145138374 cpd00205 K+ 0.034450830140485306 cpd00254 Mg 4.04776361060514E-4 cpd08053 Resazurin 4.366812227074235E-6
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